Biotechnology Research Centre Animal Laboratory
Biotechnology Research Centre (BRC) Animal Laboratory is designed to facilitate in vivo experiments. In vivo experiments are one of the most important experimental stages in medical, pharmacological and biological research, leading to clinical research. BRC animal laboratory is designed to facilitate husbandry of small laboratory animals in addition to support in vivo experiments. The laboratory is designed in accordance with guidelines including CCAC Guide to the Care and Use of Experimental Animals, ILAR Guide for the Care and Use of Laboratory Animals, and NIH Vivarium Design Policy and Guidelines. We have a policy to run a facility that has high standards for the welfare of laboratory animals and safety of laboratory personnel.
Biotechnology Research Centre Microbiology Laboratory
Biotechnology Research Centre Microbiology Laboratory is a dedicated and active laboratory facility for research in microbiology. The laboratory is part of a multidisciplinary laboratory complex. Broad range of research projects including MSc and PhD projects are supported by the microbiology laboratory. In addition to work on isolated microorganisms, interaction of prokaryote and eukaryotic cells in co-culture and effects of bacteria and bacterial products on cancer is studied. Virulence factors including bacterial pili and their interaction with cancer cells is among the researched topics. Clinical isolates and the effect of natural products and plant extracts on these, as well as laboratory strains is studied. Shaking and static incubators, colony counter, autoclaves and microscopes are among the frequently used equipment in the microbiology laboratory.
Biotechnology Research Centre Electrophysiology and Imaging Laboratory
Biotechnology Research Center Electrophysiology and Imaging Laboratory is a two part laboratory specialized in electrophysiological and imaging studies. The first part of the laboratory is designed to accommodate two fully equipped patch-clamp rig systems to facilitate electrophysiological studies. These patch clamp systems can be used to study essential electrical activity in excitable cells or tissues such as neurons and cardiomyocytes, and cancer cells. These systems also important in pharmacological studies. The second part of the laboratory accommodates up to 3 imaging system such as confocal microscopy and live imaging stations. These systems are important in immunohistochemistry, immunocytochemistry and live functional studies using specialized indicator dyes.
Biotechnology Research Centre Cell Culture Laboratory
Biotechnology Research Center Cell Culture Laboratory is equipped with normoxic and hypoxic cell culture incubators, biosafety cabinets, fully equipped fluorescent (broad spectrum) microscopy system, cryogenic cell/tissue storage tank, ultra-freezer (-80oC), and metastatic cell behaviour study systems including a microplate reader (Elisa reader). This fully functional and highly active laboratory facility is being used in cell culture, pharmacological, and metastatic cell behaviour experiments. The laboratory contains a cell bank that is used in the long time storage of cell lines including human breast cancer cell models (MDA-MB-231 and MCF-7) and rat prostate cancer cell models (Mat-LyLu and AT2). In addition, the BRC laboratory supports other laboratories by supplying working material.
Biotechnology Research Center Molecular and Wet Laboratory
The main function of Biotechnology Research Centre Molecular and Wet Laboratory is to facilitate molecular studies. The laboratory is divided into several functional working stations according to subject material and contamination risks. These working stations include solution preparation station (pH meter, magnetic stirrer, distilled water maker etc.) to prepare solutions and pharmacologic agents; protein work station (refrigerated centrifuge, SDS electrophoresis system, Western blotting system, and chemiluminescence imaging systems) for protein isolation and expression studies; DNA work station (Nano spectrophotometer, centrifuge, heating block, etc.) for cDNA synthesis; PCR work station (realtime PCR rotary cycler, UV imaging system, horizontal electrophoresis system) for nucleotide expression studies; RNA work station (centrifuge, heating block, etc.) for RNA isolation and expression studies.